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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXCL8/IL-8 Polyclonal antibody (31533-1-AP)

    WB analysis using 31533-1-AP

    Untreated, PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 31533-1-AP (CXCL8/IL-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CXCL8/IL-8 Polyclonal antibody (31533-1-AP)

    IHC staining of human tonsillitis using 31533-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31533-1-AP (CXCL8/IL-8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CXCL8/IL-8 Polyclonal antibody (31533-1-AP)

    IHC staining of human tonsillitis using 31533-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31533-1-AP (CXCL8/IL-8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CXCL8/IL-8 Polyclonal antibody (31533-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 31533-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31533-1-AP (CXCL8/IL-8 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CXCL8/IL-8 Polyclonal antibody (31533-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 31533-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31533-1-AP (CXCL8/IL-8 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CXCL8/IL-8 Polyclonal antibody

CXCL8/IL-8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31533-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CXCL8, IL8, IL-8, CXCL 8, C-X-C motif chemokine 8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells
Positive IHC detected inhuman tonsillitis tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31533-1-AP targets CXCL8/IL-8 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 8
Calculated molecular weight 99 aa, 11 kDa
Observed molecular weight11 kDa
GenBank accession numberBC013615
Gene Symbol IL-8
Gene ID (NCBI) 3576
ENSEMBL Gene IDENSG00000169429
RRIDAB_3670024
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP10145
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin 8 (IL-8), also known as CXCL8, is a CXC chemokine family member. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression. The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa).

Protocols

Product Specific Protocols
WB protocol for CXCL8/IL-8 antibody 31533-1-APDownload protocol
IHC protocol for CXCL8/IL-8 antibody 31533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols