CXCL8,IL8 Recombinant antibody, PBS Only (Capture)

Sandwich ELISA standard curve of MP00830-3, Human CXCL8/IL-8 Recombinant Matched Antibody Pair - PBS only. 83903-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0152. 83903-1-PBS was HRP conjugated as the detection antibody. Range: 2.73-175 pg/mL.

Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 or 5 days. For the PBMC supernatant cultured for 3 days, the mean CXCL8/IL-8 concentration was determined to be 4.46 ng/mL in unstimulated PBMC supernatant, 252.88 ng/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean CXCL8/IL-8 concentration was determined to be 317.16 ng/mL in PHA stimulated PBMC supernatant.

Serum of sixteen individual healthy human donors was measured. The CXCL8/IL-8 concentration of detected samples was determined to be 29.8 pg/mL with a range of 8.9 - 104.1 pg/mL.