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- KD/KO Validated
CXCR1 Polyclonal antibody
CXCR1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 55450-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IF | See 1 publications below |
Product Information
55450-1-AP targets CXCR1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 8 receptor, alpha |
| Calculated molecular weight | 40 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | NM_000634 |
| Gene symbol | CXCR1 |
| Gene ID (NCBI) | 3577 |
| RRID | AB_11182706 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CXCR1, also named CMKAR1, IL8RA, and CD181, belongs to the G-protein coupled receptor 1 family. It is a receptor to interleukin-8, a powerful neutrophil chemotactic factor. The binding of IL-8 to the receptor causes activation of neutrophils. CXCR1 binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity. This antibody is specific to CXCR1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CXCR1 antibody 55450-1-AP | Download protocol |
| IF protocol for CXCR1 antibody 55450-1-AP | Download protocol |
| IP protocol for CXCR1 antibody 55450-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis CXCR1 drives the pathogenesis of EAE and ARDS via boosting dendritic cells-dependent inflammation | ||
Cell Rep Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism
| ||
Lung Cancer Combination therapy with anti-programmed cell death 1 antibody plus angiokinase inhibitor exerts synergistic antitumor effect against malignant mesothelioma via tumor microenvironment modulation | ||
Cytokine IL-8 exacerbates alcohol-induced fatty liver disease via the Akt/HIF-1α pathway in human IL-8-expressing mice. | ||
J Agric Food Chem Oral Intervention of Narirutin Ameliorates the Allergic Response of Ovalbumin Allergy | ||
Mol Immunol IL-8 exacerbates CCl4-induced liver fibrosis in human IL-8-expressing mice via the PI3K/Akt/HIF-1α pathway |
