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Validation Data Gallery

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  • Western Blot (WB) analysis of MOLT-4 cells using CXCR3B-specific Monoclonal antibody (60065-1-Ig)

    WB analysis of MOLT-4 using 60065-1-Ig (same clone as 60065-1-PBS)

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CXCR3B-specific Monoclonal antibody (60065-1-Ig)

    WB analysis of Jurkat using 60065-1-Ig (same clone as 60065-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using CXCR3B-specific Monoclonal antibody (60065-1-Ig)

    WB analysis of K-562 using 60065-1-Ig (same clone as 60065-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using CXCR3B-specific Monoclonal antibody (60065-1-Ig)

    WB analysis of U-937 using 60065-1-Ig (same clone as 60065-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using CXCR3B-specific Monoclonal antibody (60065-1-Ig)

    WB analysis of HT-29 using 60065-1-Ig (same clone as 60065-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CXCR3B-specific Monoclonal antibody (60065-1-Ig)

    IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CXCR3B-specific Monoclonal antibody (60065-1-Ig)

    IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CXCR3B-specific Monoclonal antibody (60065-1-Ig)

    IHC staining of human brain using 60065-1-Ig (same clone as 60065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CXCR3B-specific Monoclonal antibody (60065-1-Ig)

    IHC staining of human liver cancer using 60065-1-Ig (same clone as 60065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CXCR3B-specific Monoclonal antibody (60065-1-Ig)

    IHC staining of human liver cancer using 60065-1-Ig (same clone as 60065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CXCR3B-specific Monoclonal antibody (60065-1-Ig)

    IHC staining of human brain using 60065-1-Ig (same clone as 60065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CXCR3B-specific Monoclonal antibody (60065-1-Ig)

    IF Staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CXCR3B-specific antibody (60065-1-Ig, Clone: 1B2D6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CXCR3B-specific Monoclonal antibody, PBS Only

CXCR3B-specific Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60065-1-PBS
Clone No.1B2D6

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CXCR3, CMKAR3, CKR-L2, CKR L2, CD183

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60065-1-PBS targets CXCR3B-specific in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) receptor 3
Calculated molecular weight 46 kDa
Observed molecular weight43 kDa
GenBank accession numberAF469635
Gene Symbol CXCR3
Gene ID (NCBI) 2833
RRIDAB_2292376
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49682
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.