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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CXCR5 Polyclonal antibody

CXCR5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28239-1-AP

Synonyms

MDR 15, CXCR 5, CXC R5, CD185, Burkitt lymphoma receptor 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXCR5 Polyclonal antibody (28239-1-AP)

    WB analysis using 28239-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28239-1-AP (CXCR5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CXCR5 Polyclonal antibody (28239-1-AP)

    WB analysis of Jurkat using 28239-1-AP

    WB result of CXCR5 antibody (28239-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CXCR5 transfected Jurkat cells.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CXCR5 Polyclonal antibody (28239-1-AP)

    IHC staining of mouse brain using 28239-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28239-1-AP (CXCR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, K-562 cells, mouse spleen tissue, rat spleen tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28239-1-AP targets CXCR5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCR5 fusion protein Ag28271 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) receptor 5
Calculated molecular weight 42 kDa
Observed molecular weight33 kDa
GenBank accession numberNM_001716
Gene symbol CXCR5
Gene ID (NCBI) 643
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Initially discovered in 1992 as Burkitt's lymphoma receptor 1, CXCR5 (chemokine receptor 5, also known as CD185) is a seven-transmembrane G protein-coupled receptor (GPCR) protein (PMID: 32994482). CXCR5 is essential for naïve B-cell migration to and maturation in the lymph nodes and spleen, as well as for cluster of differentiation 4 T-cell (TFH and TCM) homing and interaction with B cells (PMID: 21471443). Moreover, CXCR5 is involved in the regulation of neural stem cells (NSC) and neuronal regeneration in the mouse brain, neuronal differentiation, neurogenesis, gliogenesis, and synaptogenesis (PMID: 37460877).

Protocols

Product Specific Protocols
WB protocol for CXCR5 antibody 28239-1-APDownload protocol
IHC protocol for CXCR5 antibody 28239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols