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  • Featured Product
  • KD/KO Validated

CXCR7 Polyclonal antibody, PBS Only

CXCR7 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20423-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using CXCR7 Polyclonal antibody (20423-1-AP)

    WB analysis of Raji using 20423-1-AP (same clone as 20423-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using CXCR7 Polyclonal antibody (20423-1-AP)

    WB analysis of K-562 using 20423-1-AP (same clone as 20423-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CXCR7 Polyclonal antibody (20423-1-AP)

    WB analysis of Jurkat using 20423-1-AP (same clone as 20423-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using CXCR7 Polyclonal antibody (20423-1-AP)

    WB analysis of mouse thymus using 20423-1-AP (same clone as 20423-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HUVEC cells using CXCR7 Polyclonal antibody (20423-1-AP)

    IP experiment of HUVEC using 20423-1-AP (same clone as 20423-1-PBS)

    IP result of anti-CXCR7 (IP:20423-1-AP, 3ug; Detection:20423-1-AP 1:500) with HUVEC cells lysate 1400ug. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CXCR7 Polyclonal antibody (20423-1-AP)

    IHC staining of mouse brain using 20423-1-AP (same clone as 20423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20423-1-AP (CXCR7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CXCR7 Polyclonal antibody (20423-1-AP)

    IHC staining of mouse brain using 20423-1-AP (same clone as 20423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20423-1-AP (CXCR7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20423-1-PBS targets CXCR7 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCR7 fusion protein Ag14247 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) receptor 7
Calculated molecular weight 362 aa, 41 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC036661
Gene symbol CXCR7
Gene ID (NCBI) 57007
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).