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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXCR7 Recombinant antibody (83927-1-RR)

    WB analysis using 83927-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83927-1-RR (CXCR7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CXCR7 Recombinant antibody (83927-1-RR)

    IF Staining of Jurkat using 83927-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CXCR7 antibody (83927-1-RR, Clone: 240842C9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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CXCR7 Recombinant antibody

CXCR7 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83927-1-RR
Clone No.240842C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ACKR3, 240842C9, C-X-C chemokine receptor type 7, CMKOR1, Chemokine orphan receptor 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, Raji cells, U-87 MG cells
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83927-1-RR targets CXCR7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CXCR7 fusion protein Ag14247 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) receptor 7
Calculated molecular weight 362 aa, 41 kDa
Observed molecular weight50 kDa
GenBank accession numberBC036661
Gene Symbol CXCR7
Gene ID (NCBI) 57007
RRIDAB_3671507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP25106
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a G-protein coupled receptor family member. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as a coreceptor with CXCR4 for a restricted number of HIV isolates. The expression of CXCR7 has been associated with cardiac development, tumor growth, and progression.

Protocols

Product Specific Protocols
WB protocol for CXCR7 antibody 83927-1-RRDownload protocol
IF protocol for CXCR7 antibody 83927-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols