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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXXC5 Recombinant antibody (83940-7-RR)

    WB analysis using 83940-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83940-7-RR (CXXC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83940-7-RR

    Affinity and Kinetic Characterization of 83940-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83940-7-RR against Human CXXC5 were performed. The affinity constant is 5.73 nM.

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CXXC5 Recombinant antibody

CXXC5 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83940-7-RR
Clone No.241034A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Putative MAPK-activating protein PM08, HSPC195, CXXC-type zinc finger protein 5, CXXC finger 5, CF5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, K-562 cells, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83940-7-RR targets CXXC5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CXXC5 fusion protein Ag9733 相同性解析による交差性が予測される生物種
Full Name CXXC finger 5
Calculated molecular weight322aa,33 kDa; 227aa,24 kDa
Observed molecular weight 33-40 kDa
GenBank accession numberBC017439
Gene Symbol CXXC5
Gene ID (NCBI) 51523
RRIDAB_3671522
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ7LFL8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CXXC5 (CXXC-type zinc finger protein 5) is also named as HSPC195, RINF and TCCCIA00297.CXXC5 is a member of the CXXC-type zinc-finger protein family. CXXC5 is a short protein (322 amino acids in length) that does not have any catalytic domain, but is able to bind to DNA and act as a transcription factor and epigenetic factor through protein-protein interactions. In addition, CXXC5 is capable of regulating various signal transduction processes, including the TGF-β, Wnt and ATM-p53 pathways, thereby acting as a novel and crucial signaling coordinator. CXXC5 plays an important role in embryonic development and adult tissue homeostasis by regulating cell proliferation, differentiation and apoptosis (PMID: 30479059). CXXC5 has two isoforms of 33 kDa and 24 kDa (PMID: 25805812).

Protocols

Product Specific Protocols
WB protocol for CXXC5 antibody 83940-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols