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KD/KO Validated

CYB5B Polyclonal antibody

Name: CYB5B Polyclonal antibody
Brand: Proteintech
SKU: 15469-1-AP

CYB5B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15469-1-AP

Synonyms

OMB5, Cytochrome b5 type B, CYPB5M, CYB5M, CYB5 M

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 15469-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 15469-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:4000 incubated at 4 degree celsius over night.
WB analysis of human liver using 15469-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 15469-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human adrenal gland using 15469-1-AP
human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15469-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15469-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15469-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 15469-1-AP
IP result of anti-CYB5B (IP:15469-1-AP, 3ug; Detection:15469-1-AP 1:500) with mouse lung tissue lysate 4000ug.
IHC staining of human testis using 15469-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15469-1-AP (CYB5B antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 15469-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15469-1-AP (CYB5B antibody) at dilution of 1:100 (under 40x lens).
IHC staining of mouse testis using 15469-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15469-1-AP (CYB5B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 15469-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15469-1-AP (CYB5B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15469-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15469-1-AP (CYB5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 15469-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue, human liver tissue, mouse lung tissue, human adrenal gland tissue, rat liver tissue, rat lung tissue
Positive IP detected in	mouse lung tissue
Positive IHC detected in	human testis tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 1 publications below

Product Information

15469-1-AP targets CYB5B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CYB5B fusion protein Ag7771 相同性解析による交差性が予測される生物種
Full Name	cytochrome b5 type B (outer mitochondrial membrane)
Calculated molecular weight	16 kDa
Observed molecular weight	20-21 kDa
GenBank accession number	BC004373
Gene symbol	CYB5B
Gene ID (NCBI)	80777
RRID	AB_2230349
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CYB5B(Cytochrome b5 type B) is also named as CYB5M, OMB5 and belongs to the cytochrome b5 family. It is a 21 kDa membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. This protein is expressed on the plasma membrane of lymphoma cells but not normal lymphocytes, reactive lymphocytes or bone marrow precursor cells(PMID:20100355). The full length protein has a propeptide with 11 amino acids.

Protocols

Product Specific Protocols
WB protocol for CYB5B antibody 15469-1-AP	Download protocol
IHC protocol for CYB5B antibody 15469-1-AP	Download protocol
IF protocol for CYB5B antibody 15469-1-AP	Download protocol
IP protocol for CYB5B antibody 15469-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Chem Biol Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Authors - Paige Dickson KD Validated View Article
mouse	WB	PLoS Genet Divergent role of Mitochondrial Amidoxime Reducing Component 1 (MARC1) in human and mouse Authors - Eriks Smagris View Article
human	IHC	Front Mol Biosci Comprehensive Analysis of mTORC1 Signaling Pathway-Related Genes in the Prognosis of HNSCC and the Response to Chemotherapy and Immunotherapy. Authors - Zhao Ding View Article
human	WB	J Biol Chem Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome Authors - Hana Nůsková View Article
human	WB	J Cell Biol Mechanosensitive super-enhancers regulate genes linked to atherosclerosis in endothelial cells Authors - Jin Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYB5B Polyclonal antibody

CYB5B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 15469-1-AP

WB analysis using 15469-1-AP

WB analysis of human liver using 15469-1-AP

WB analysis of mouse lung using 15469-1-AP

WB analysis of human adrenal gland using 15469-1-AP

WB analysis of HepG2 using 15469-1-AP

WB analysis of HepG2 using 15469-1-AP

WB analysis of mouse liver using 15469-1-AP

IP experiment of mouse lung using 15469-1-AP

IHC staining of human testis using 15469-1-AP

IHC staining of human testis using 15469-1-AP

IHC staining of mouse testis using 15469-1-AP

IHC staining of mouse testis using 15469-1-AP

IF Staining of HepG2 using 15469-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190.

Divergent role of Mitochondrial Amidoxime Reducing Component 1 (MARC1) in human and mouse

Comprehensive Analysis of mTORC1 Signaling Pathway-Related Genes in the Prognosis of HNSCC and the Response to Chemotherapy and Immunotherapy.

Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome

Mechanosensitive super-enhancers regulate genes linked to atherosclerosis in endothelial cells