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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYB5R1 Polyclonal antibody

CYB5R1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11807-1-AP

Synonyms

NADH-cytochrome b5 reductase 1, NADH cytochrome b5 reductase 1, Humb5R2, EC:1.6.2.2, cytochrome b5 reductase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using CYB5R1 Polyclonal antibody (11807-1-AP)

    WB analysis of human placenta using 11807-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11807-1-AP (CYB5R1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CYB5R1 Polyclonal antibody (11807-1-AP)

    WB analysis of PC-3 using 11807-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11807-1-AP (CYB5R1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CYB5R1 Polyclonal antibody (11807-1-AP)

    WB analysis of human brain using 11807-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11807-1-AP (CYB5R1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human placenta tissue using CYB5R1 Polyclonal antibody (11807-1-AP)

    IP experiment of human placenta using 11807-1-AP

    IP result of anti-CYB5R1 (IP:11807-1-AP, 4ug; Detection:11807-1-AP 1:1000) with human placenta tissue lysate 1520ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CYB5R1 Polyclonal antibody (11807-1-AP)

    IHC staining of human breast cancer using 11807-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11807-1-AP (CYB5R1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using CYB5R1 Polyclonal antibody (11807-1-AP)

    IF Staining of PC-3 using 11807-1-AP

    Immunofluorescent analysis of PC-3 cells using 11807-1-AP (CYB5R1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman placenta tissue, human brain tissue, PC-3 cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

11807-1-AP targets CYB5R1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYB5R1 fusion protein Ag2475 相同性解析による交差性が予測される生物種
Full Name cytochrome b5 reductase 1
Calculated molecular weight 305 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC018732
Gene symbol CYB5R1
Gene ID (NCBI) 51706
RRIDAB_2089968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CYB5R1 antibody 11807-1-APDownload protocol
IHC protocol for CYB5R1 antibody 11807-1-APDownload protocol
IF protocol for CYB5R1 antibody 11807-1-APDownload protocol
IP protocol for CYB5R1 antibody 11807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Free Radic Res

Iron deficiency aggravates DMNQ-induced cytotoxicity via redox cycling in kidney-derived cells

Authors - Daisaku Yoshihara
View Article
humanWB

J Transl Med

Transcriptomics-proteomics Integration reveals alternative polyadenylation driving inflammation-related protein translation in patients with diabetic nephropathy

Authors - Tingting Zhao
View Article