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KD/KO Validated

NOX2 Polyclonal antibody

Name: NOX2 Polyclonal antibody
Brand: Proteintech
SKU: 19013-1-AP

NOX2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF-P, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Publications

171

Review Product

Cat no : 19013-1-AP

Synonyms

CYBB, Heme-binding membrane glycoprotein gp91phox, EC:1.-.-.-, Cytochrome b-245 heavy chain, CGD91-phox

Validation Data Gallery

Filter:

AllWBIHCIF-PFC (Intra)

WB analysis using 19013-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 19013-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 19013-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human plasma using 19013-1-AP
human serum were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 19013-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 19013-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 19013-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 19013-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 19013-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 19013-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 19013-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 19013-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 19013-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 19013-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 19013-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 19013-1-AP (NOX2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 19013-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NOX2 antibody (19013-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of RAW 264.7 using 19013-1-AP
1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human NOX2 (19013-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 19013-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse spleen tissue, Jurkat cells, Raji cells, human plasma, rat spleen tissue
Positive IHC detected in	human tonsillitis tissue, mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue, human tonsillitis tissue
Positive FC (Intra) detected in	RAW 264.7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:8000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 148 publications below
IHC	See 16 publications below
IF	See 17 publications below
CoIP	See 1 publications below

Product Information

19013-1-AP targets NOX2 in WB, IHC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NOX2 fusion protein Ag5536 相同性解析による交差性が予測される生物種
Full Name	cytochrome b-245, beta polypeptide
Calculated molecular weight	577 aa, 65 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC032720
Gene symbol	NOX2
Gene ID (NCBI)	1536
RRID	AB_2833044
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa(PMID: 17587483) to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781).

Protocols

Product Specific Protocols
WB protocol for NOX2 antibody 19013-1-AP	Download protocol
IHC protocol for NOX2 antibody 19013-1-AP	Download protocol
IF protocol for NOX2 antibody 19013-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Circulation Megakaryocytic Leukemia 1 (MKL1) Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury. Authors - Yu Liming View Article
mouse	WB,IHC	Cell Host Microbe Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation Authors - Zhenyu Tian View Article
		Sci Adv Sphingolipid biosynthesis is essential for metabolic rewiring during TH17 cell differentiation Authors - Thiruvaimozhi Abimannan View Article
human	WB,IF	Redox Biol Silencing COX-2 blocks PDK1/TRAF4-induced AKT activation to inhibit fibrogenesis during skeletal muscle atrophy. Authors - Hongtao Chen View Article
pig	IF,WB	Redox Biol Nox2 impairs VEGF-A-induced angiogenesis in placenta via mitochondrial ROS-STAT3 pathway. Authors - Chengjun Hu KD Validated View Article
rat	WB	Redox Biol RND3 attenuates oxidative stress and vascular remodeling in spontaneously hypertensive rat via inhibiting ROCK1 signaling. Authors - Nan Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NOX2 Polyclonal antibody

NOX2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 19013-1-AP

WB analysis of mouse spleen using 19013-1-AP

WB analysis of Raji using 19013-1-AP

WB analysis of human plasma using 19013-1-AP

WB analysis of mouse spleen using 19013-1-AP

WB analysis using 19013-1-AP

IHC staining of human tonsillitis using 19013-1-AP

IHC staining of human tonsillitis using 19013-1-AP

IHC staining of human tonsillitis using 19013-1-AP

IHC staining of mouse spleen using 19013-1-AP

IHC staining of human tonsillitis using 19013-1-AP

IHC staining of human tonsillitis using 19013-1-AP

IHC staining of mouse spleen using 19013-1-AP

IHC staining of mouse spleen using 19013-1-AP

IF Staining of human tonsillitis using 19013-1-AP

IF Staining of mouse brain using 19013-1-AP

FC experiment of RAW 264.7 using 19013-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Megakaryocytic Leukemia 1 (MKL1) Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury.

Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation

Sphingolipid biosynthesis is essential for metabolic rewiring during TH17 cell differentiation

Silencing COX-2 blocks PDK1/TRAF4-induced AKT activation to inhibit fibrogenesis during skeletal muscle atrophy.

Nox2 impairs VEGF-A-induced angiogenesis in placenta via mitochondrial ROS-STAT3 pathway.

RND3 attenuates oxidative stress and vascular remodeling in spontaneously hypertensive rat via inhibiting ROCK1 signaling.