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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYFIP2 Recombinant antibody

CYFIP2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240909E3

Cat no : 83845-3-RR

Synonyms

CYFIP1/2, 240909E3, p53-inducible protein 121, p53 inducible protein 121, KIAA1168

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CYFIP2 Recombinant antibody (83845-3-RR)

    IF Staining of Jurkat using 83845-3-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CYFIP2 antibody (83845-3-RR, Clone: 240909E3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of Jurkat cells using CYFIP2 Recombinant antibody (83845-3-RR)

    FC experiment of Jurkat using 83845-3-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Cyfip2 Recombinant Antibody (83845-3-RR, Clone:240909E3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83845-3-RR is CYFIP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CYFIP2 fusion protein Ag8749 相同性解析による交差性が予測される生物種
Full Name cytoplasmic FMR1 interacting protein 2
Calculated molecular weight 148 kDa
GenBank accession numberBC011762
Gene symbol CYFIP2
Gene ID (NCBI) 26999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYFIP1/2 belongs to the CYFIP family and is involved in T-cell adhesion and p53-dependent induction of apoptosis. CYFIP1 contains 1,253 amino acids and shares 88% sequence identity with CYFIP2. CYFIP1 and CYFIP2 are members of a highly conserved protein family and share approximately 99% sequence identity with their mouse orthologs. CYFIP1 has been shown to interact with the small GTPase RAC1, which is implicated in development and maintenance of neuronal structures. Consistent with FMRP and RAC1 localization in dendritic fine structures, CYFIP1/2 are present in synaptosomal extracts. This antibody can recognize both CYFIP1 and CYFIP2.

Protocols

Product Specific Protocols
IF protocol for CYFIP2 antibody 83845-3-RRDownload protocol
FC protocol for CYFIP2 antibody 83845-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols