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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYLD Monoclonal antibody (66858-1-Ig)

    WB analysis using 66858-1-Ig (same clone as 66858-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66858-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CYLD Monoclonal antibody (66858-1-Ig)

    WB analysis using 66858-1-Ig (same clone as 66858-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66858-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CYLD Monoclonal antibody (66858-1-Ig)

    WB analysis of HEK-293 using 66858-1-Ig (same clone as 66858-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66858-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using CYLD Monoclonal antibody (66858-1-Ig)

    WB analysis of A431 using 66858-1-Ig (same clone as 66858-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66858-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CYLD Monoclonal antibody (66858-1-Ig)

    IF Staining of SH-SY5Y using 66858-1-Ig (same clone as 66858-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CYLD antibody (66858-1-Ig, Clone: 1G2F4 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66858-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CYLD Monoclonal antibody (66858-1-Ig)

    IF Staining of SH-SY5Y using 66858-1-Ig (same clone as 66858-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CYLD antibody (66858-1-Ig, Clone: 1G2F4 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66858-1-PBS in a different storage buffer formulation.

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CYLD Monoclonal antibody, PBS Only

CYLD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66858-1-PBS
Clone No.1G2F4

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CDMT, CYLD, CYLD1, CYLDI, Deubiquitinating enzyme CYLD, EAC, FLJ20180, FLJ31664, FLJ78684, HSPC057, KIAA0849, MFT, MFT1, SBS, TEM, Ubiquitin thiolesterase CYLD, USPL2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66858-1-PBS targets CYLD in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CYLD fusion protein Ag28333 相同性解析による交差性が予測される生物種
Full Name cylindromatosis (turban tumor syndrome)
Calculated molecular weight 107 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC012342
Gene Symbol CYLD
Gene ID (NCBI) 1540
RRIDAB_2882197
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQC7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CYLD, also named as CYLD1, belongs to the peptidase C67 family. It is the protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. CYLD has endodeubiquitinase activity and plays an important role in the regulation of pathways leading to NF-kappa-B activation. CYLD contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. It is a negative regulator of Wnt signaling. CYLD inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. CYLD plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. It is required for normal cell cycle progress and normal cytokinesis. CYLD inhibits nuclear translocation of NF-kappa-B and plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. It is dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. CYLD negatively regulates TNFRSF11A signaling and osteoclastogenesis.