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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP11B2 Recombinant antibody

CYP11B2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240811E7

Cat no : 83930-1-RR

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using CYP11B2 Recombinant antibody (83930-1-RR)

    WB analysis of SKOV-3 using 83930-1-RR

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 83930-1-RR (CYP11B2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83930-1-RR targets CYP11B2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 11, subfamily B, polypeptide 2
Calculated molecular weight 58 kDa
Observed molecular weight 58-60 kDa
GenBank accession numberNM_000498
Gene symbol CYP11B2
Gene ID (NCBI) 1585
RRIDAB_3671511
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP11B2 (Cytochrome P450 11B2), also known as aldosterone synthase, is a member of the cytochrome P450 superfamily of enzymes. CYP11B2 can only catalyze 11-deoxycorticosterone oxidation to produce corticosterone and further hydroxylation to ALD. CYP11B2 is an enzyme required for cortisol biosynthesis and is specifically expressed in aldosterone-producing tissue of the adrenal cortex and is overexpressed in aldosterone-producing adenomas (APA).

Protocols

Product Specific Protocols
WB protocol for CYP11B2 antibody 83930-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols