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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP19A1 Polyclonal antibody (28379-1-AP)

    WB analysis using 28379-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28379-1-AP (CYP19A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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CYP19A1 Polyclonal antibody

CYP19A1 Polyclonal Antibody for WB, ELISA
Cat No. 28379-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHL-60 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28379-1-AP targets CYP19A1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP19A1 fusion protein Ag29004 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 19, subfamily A, polypeptide 1
Observed molecular weight 58-60 kDa
GenBank accession numberBC035959
Gene Symbol CYP19A1
Gene ID (NCBI) 1588
RRIDAB_3669657
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11511
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510).

Protocols

Product Specific Protocols
WB protocol for CYP19A1 antibody 28379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols