Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of mouse liver using 19936-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 19936-1-AP (CYP1A2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 19936-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 19936-1-AP (CYP1A2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 19936-1-AP
IP result of anti-CYP1A2-Specific (IP:19936-1-AP, 4ug; Detection:19936-1-AP 1:300) with mouse liver tissue lysate 4000ug.
IHC staining of human liver using 19936-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19936-1-AP (CYP1A2-Specific Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of mouse liver using 19936-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19936-1-AP (CYP1A2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 19936-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYP1A2-Specific antibody (19936-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of HepG2 using 19936-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CYP1A2-Specific Polyclonal antibody (19936-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CYP1A2-Specific Polyclonal antibody

CYP1A2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 19936-1-AP

Publications(42)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

CYP1A2, Cytochrome P(3)450, CYPIA2, CP1A2, CP12

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (42)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 36 publications below
IHC	See 1 publications below
IF	See 7 publications below

Product Information

19936-1-AP targets CYP1A2-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	cytochrome P450, family 1, subfamily A, polypeptide 2
Calculated molecular weight	58 kDa
Observed molecular weight	58 kDa
GenBank accession number	NM_000761
Gene Symbol	CYP1A2
Gene ID (NCBI)	1544
RRID	AB_10638909
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P05177
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CYP1A2, also named as CP12; P3-450 and P450(PA), belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active of CYP1A2 is in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. It also acts in the metabolism of aflatoxin B1 and acetaminophen. CYP1A2 participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. It catalizes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP1A2.

Protocols

Product Specific Protocols
WB protocol for CYP1A2-Specific antibody 19936-1-AP	Download protocol
IHC protocol for CYP1A2-Specific antibody 19936-1-AP	Download protocol
IF protocol for CYP1A2-Specific antibody 19936-1-AP	Download protocol
IP protocol for CYP1A2-Specific antibody 19936-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Host Microbe Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice Authors - Yunong Zeng View Article
mouse	IF	Hepatology Self-renewal of hepatoblasts under chemically defined conditions by reiterative growth factor and chemical screening. Authors - Linjie Lv View Article
human	IF	Acta Biomater Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply. Authors - Kun Du View Article
human	IF	ACS Appl Mater Interfaces Modeling Colorectal Cancer-Induced Liver Portal Vein Microthrombus on a Hepatic Lobule Chip Authors - Xiaofang Du View Article
mouse	WB	Br J Pharmacol Is platelet responsiveness to clopidogrel attenuated in overweight or obese patients and why? A reverse translational study in mice. Authors - Li-Ping Jiang View Article
mouse	IF,WB	Cell Mol Gastroenterol Hepatol Aryl Hydrocarbon Receptor Activity in Hepatocytes Sensitizes to Hyperacute Acetaminophen-Induced Hepatotoxicity in Mice. Authors - Fenja A Schuran View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse liver using 19936-1-AP

WB analysis of mouse liver using 19936-1-AP

IP experiment of mouse liver using 19936-1-AP

IHC staining of human liver using 19936-1-AP

IHC staining of mouse liver using 19936-1-AP

IF Staining of HeLa using 19936-1-AP

FC experiment of HepG2 using 19936-1-AP

CYP1A2-Specific Polyclonal antibody

CYP1A2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice

Self-renewal of hepatoblasts under chemically defined conditions by reiterative growth factor and chemical screening.

Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.

Modeling Colorectal Cancer-Induced Liver Portal Vein Microthrombus on a Hepatic Lobule Chip

Is platelet responsiveness to clopidogrel attenuated in overweight or obese patients and why? A reverse translational study in mice.

Aryl Hydrocarbon Receptor Activity in Hepatocytes Sensitizes to Hyperacute Acetaminophen-Induced Hepatotoxicity in Mice.