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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP1B1 Monoclonal antibody, PBS Only

CYP1B1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G8D4

Cat no : 67033-1-PBS

Synonyms

CP1B, CYP1B1, CYPIB1, Cytochrome P450 1B1, GLC3A, P4501B1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CYP1B1 Monoclonal antibody (67033-1-Ig)

    WB analysis using 67033-1-Ig (same clone as 67033-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using CYP1B1 Monoclonal antibody (67033-1-Ig)

    WB analysis of PC-3 using 67033-1-Ig (same clone as 67033-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using CYP1B1 Monoclonal antibody (67033-1-Ig)

    WB analysis of pig brain using 67033-1-Ig (same clone as 67033-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using CYP1B1 Monoclonal antibody (67033-1-Ig)

    WB analysis of rat brain using 67033-1-Ig (same clone as 67033-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CYP1B1 Monoclonal antibody (67033-1-Ig)

    WB analysis of mouse brain using 67033-1-Ig (same clone as 67033-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67033-1-PBS targets CYP1B1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP1B1 fusion protein Ag13380 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 1, subfamily B, polypeptide 1
Calculated molecular weight 61 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC012049
Gene symbol CYP1B1
Gene ID (NCBI) 1545
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).