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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP20A1 Monoclonal antibody (67433-1-Ig)

    WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CYP20A1 Monoclonal antibody (67433-1-Ig)

    WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CYP20A1 Monoclonal antibody (67433-1-Ig)

    WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP20A1 Monoclonal antibody (67433-1-Ig)

    IHC staining of human liver cancer using 67433-1-Ig (same clone as 67433-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP20A1 Monoclonal antibody (67433-1-Ig)

    IHC staining of human liver cancer using 67433-1-Ig (same clone as 67433-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CYP20A1 Monoclonal antibody (67433-1-Ig)

    IF Staining of HeLa using 67433-1-Ig (same clone as 67433-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CYP20A1 Monoclonal antibody (67433-1-Ig)

    IF Staining of HeLa using 67433-1-Ig (same clone as 67433-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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CYP20A1 Monoclonal antibody, PBS Only

CYP20A1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67433-1-PBS
Clone No.2A9E2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CYP M, CYP20A1, Cytochrome P450 20A1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67433-1-PBS targets CYP20A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP20A1 fusion protein Ag9715 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 20, subfamily A, polypeptide 1
Calculated molecular weight 462 aa, 52 kDa
Observed molecular weight 49-52 kDa
GenBank accession numberBC020616
Gene Symbol CYP20A1
Gene ID (NCBI) 57404
RRIDAB_2882671
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6UW02
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.