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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP21A2 Monoclonal antibody

CYP21A2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G2G6

Cat no : 67421-1-Ig

Synonyms

CA21H, CAH1, CYP21, CYP21A2, CYP21B, P450c21B, Steroid 21-hydroxylase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP21A2 Monoclonal antibody (67421-1-Ig)

    WB analysis using 67421-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of PC-12 cells using CYP21A2 Monoclonal antibody (67421-1-Ig)

    WB analysis of PC-12 using 67421-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig adrenal gland tissue using CYP21A2 Monoclonal antibody (67421-1-Ig)

    WB analysis of pig adrenal gland using 67421-1-Ig

    pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CYP21A2 Monoclonal antibody (67421-1-Ig)

    IF Staining of PC-12 using 67421-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using CYP21A2 antibody (67421-1-Ig, Clone: 1G2G6 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, PC-12 cells, pig adrenal gland tissue
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67421-1-Ig targets CYP21A2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP21A2 fusion protein Ag26292 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 21, subfamily A, polypeptide 2
Calculated molecular weight 56 kDa
Observed molecular weight 53-56 kDa
GenBank accession numberNM_000500
Gene symbol CYP21A2
Gene ID (NCBI) 1589
RRIDAB_2882661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CYP21A2 antibody 67421-1-IgDownload protocol
IF protocol for CYP21A2 antibody 67421-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols