Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat thymus tissue, A549 cells, HeLa cells, A431 cells, rat lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84821-3-RR targets CYP24A1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CYP24A1 fusion protein Ag17089 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 24, subfamily A, polypeptide 1 |
| Calculated molecular weight | 514 aa, 59 kDa |
| Observed molecular weight | 51 kDa |
| GenBank accession number | BC109083 |
| Gene Symbol | CYP24A1 |
| Gene ID (NCBI) | 1591 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q07973 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CYP24A1(1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial) catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and 1-alpha,25-dihydroxyvitamin D3. Cholecalciferol is metabolized by the 25-hydroxylases (CYP2R1 and CYP27A1) in the liver before 25-hydroxycholecalciferol (25(OH)D3) relocates to the circulation (PMID:20362668). The gene CYP24A1 has three isoforms, for the transit peptide "mitochondrial domain" removed, the MW of CYP24A1 will be 55-59 kDa, 47-51 kDa, and 43 kDa. This antibody is specific to CYP24A1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP24A1 antibody 84821-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |