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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP26A1 Polyclonal antibody (28081-1-AP)

    WB analysis using 28081-1-AP

    Human placenta tissue were subjected to SDS PAGE followed by western blot with 28081-1-AP (CYP26A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CYP26A1 Polyclonal antibody (28081-1-AP)

    IHC staining of human placenta using 28081-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28081-1-AP (CYP26A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CYP26A1 Polyclonal antibody (28081-1-AP)

    IHC staining of mouse skeletal muscle using 28081-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28081-1-AP (CYP26A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CYP26A1 Polyclonal antibody (28081-1-AP)

    IHC staining of human placenta using 28081-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28081-1-AP (CYP26A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CYP26A1 Polyclonal antibody (28081-1-AP)

    IHC staining of mouse skeletal muscle using 28081-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28081-1-AP (CYP26A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CYP26A1 Polyclonal antibody (28081-1-AP)

    IF Staining of human placenta using 28081-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CYP26A1 antibody (28081-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CYP26A1 Polyclonal antibody (28081-1-AP)

    IF Staining of human placenta using 28081-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CYP26A1 antibody (28081-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CYP26A1 Polyclonal antibody

CYP26A1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 28081-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

Cytochrome P450RAI, Cytochrome P450 26A1, CYP26, CP26

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28081-1-AP targets CYP26A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP26A1 fusion protein Ag24898 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 26, subfamily A, polypeptide 1
Calculated molecular weight 497 aa, 56 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC157063
Gene Symbol CYP26A1
Gene ID (NCBI) 1592
RRIDAB_3669643
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43174
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP26A1 (Cytochrome P450 Family 26 Subfamily A Member 1), also known as Cytochrome P450 26A1, CP26, CYP26, P450RAI, and P450RAI1, is a member of the cytochrome P450 superfamily of enzymes, involved in retinoic acid (RA) metabolism and synthesis of cholesterol, steroids, and other lipids. CYP26A1 is essential for normal hindbrain patterning, vertebral identity, and development of posterior structures(PMID: 11157778).

Protocols

Product Specific Protocols
WB protocol for CYP26A1 antibody 28081-1-APDownload protocol
IHC protocol for CYP26A1 antibody 28081-1-APDownload protocol
IF protocol for CYP26A1 antibody 28081-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols