• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CYP27A1 Monoclonal antibody, PBS Only

CYP27A1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G3F7

Cat no : 67045-1-PBS

Synonyms

CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Caco-2 cells using CYP27A1 Monoclonal antibody (67045-1-Ig)

    WB analysis of Caco-2 using 67045-1-Ig (same clone as 67045-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CYP27A1 Monoclonal antibody (67045-1-Ig)

    WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

    WB result of CYP27A1 antibody (67045-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP27A1 transfected HepG2 cells. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CYP27A1 Monoclonal antibody (67045-1-Ig)

    WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using CYP27A1 Monoclonal antibody (67045-1-Ig)

    WB analysis of HT-29 using 67045-1-Ig (same clone as 67045-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using CYP27A1 Monoclonal antibody (67045-1-Ig)

    WB analysis of rat liver using 67045-1-Ig (same clone as 67045-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CYP27A1 Monoclonal antibody (67045-1-Ig)

    WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using CYP27A1 Monoclonal antibody (67045-1-Ig)

    WB analysis of HT-29 using 67045-1-Ig (same clone as 67045-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP27A1 Monoclonal antibody (67045-1-Ig)

    IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP27A1 Monoclonal antibody (67045-1-Ig)

    IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CYP27A1 Monoclonal antibody (67045-1-Ig)

    IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CYP27A1 Monoclonal antibody (67045-1-Ig)

    IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67045-1-PBS targets CYP27A1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP27A1 fusion protein Ag7746 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 27, subfamily A, polypeptide 1
Calculated molecular weight 60 kDa
Observed molecular weight 54-60 kDa
GenBank accession numberBC051851
Gene symbol CYP27A1
Gene ID (NCBI) 1593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.