CYP2A6 Polyclonal antibody

Name: CYP2A6 Polyclonal antibody
Brand: Proteintech
SKU: 21721-1-AP

CYP2A6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21721-1-AP

Synonyms

Coumarin 7 hydroxylase, CYP2A, CYP2A3, CYP2A6, CYPIIA6, Cytochrome P450 2A6, Cytochrome P450 IIA3, Cytochrome P450(I), P450C2A, P450PB

Validation Data Gallery

Filter:

AllWBIHC

WB analysis using 21721-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21721-1-AP (CYP2A6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 21721-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 21721-1-AP (CYP2A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21721-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21721-1-AP (CYP2A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 21721-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21721-1-AP (CYP2A6 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 21721-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21721-1-AP (CYP2A6 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human liver cancer using 21721-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21721-1-AP (CYP2A6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 21721-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21721-1-AP (CYP2A6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 21721-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, HepG2 cells, mouse lung tissue, rat liver tissue
Positive IHC detected in	human liver tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

The immunogen of 21721-1-AP is CYP2A6 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CYP2A6 fusion protein Ag16377 相同性解析による交差性が予測される生物種
Full Name	cytochrome P450, family 2, subfamily A, polypeptide 6
Calculated molecular weight	494 aa, 57 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC096253
Gene symbol	CYP2A6
Gene ID (NCBI)	1548
RRID	AB_10791886
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CYP2A6 antibody 21721-1-AP	Download protocol
IHC protocol for CYP2A6 antibody 21721-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Biochem Pharmacol Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells. Authors - Ashley A Untereiner View Article
human,mouse	WB	Xenobiotica Comparison of mouse and human cytochrome P450 mediated-drug metabolising activities in hepatic and extrahepatic microsomes. Authors - Shotaro Uehara View Article
human	WB	Sci Rep Extracellular vesicles released from macrophages modulates interleukin-1β in astrocytic and neuronal cells Authors - Sunitha Kodidela View Article
mouse	WB	Front Toxicol E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis Authors - Bayan Alakhtar View Article
mouse		Biochem Biophys Res Commun Circadian rhythms in CYP2A5 expression underlie the time-dependent effect of tegafur on breast cancer Authors - Yuya Yoshida View Article
		Biochem Biophys Res Commun Circadian rhythms in CYP2A5 expression underlie the time-dependent effect of tegafur on breast cancer Authors - Yuya Yoshida View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP2A6 Polyclonal antibody

CYP2A6 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 21721-1-AP

WB analysis of mouse liver using 21721-1-AP

WB analysis of HepG2 using 21721-1-AP

IHC staining of human liver using 21721-1-AP

IHC staining of human liver using 21721-1-AP

IHC staining of human liver cancer using 21721-1-AP

IHC staining of human liver cancer using 21721-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells.

Comparison of mouse and human cytochrome P450 mediated-drug metabolising activities in hepatic and extrahepatic microsomes.

Extracellular vesicles released from macrophages modulates interleukin-1β in astrocytic and neuronal cells

E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis

Circadian rhythms in CYP2A5 expression underlie the time-dependent effect of tegafur on breast cancer

Circadian rhythms in CYP2A5 expression underlie the time-dependent effect of tegafur on breast cancer