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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP2C9 Polyclonal antibody

CYP2C9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16355-1-AP

Synonyms

(R) limonene 6 monooxygenase, (S) limonene 6 monooxygenase, (S) limonene 7 monooxygenase, CPC9, CYP2C, CYP2C10, CYP2C9, CYPIIC9, Cytochrome P 450MP, Cytochrome P450 2C9, Cytochrome P450 MP 4, Cytochrome P450 MP 8, Cytochrome P450 PB 1, P450IIC9, S mephenytoin 4 hydroxylase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP2C9 Polyclonal antibody (16355-1-AP)

    WB analysis using 16355-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16355-1-AP (CYP2C9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using CYP2C9 Polyclonal antibody (16355-1-AP)

    WB analysis of SMMC-7721 using 16355-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16355-1-AP (CYP2C9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP2C9 Polyclonal antibody (16355-1-AP)

    IHC staining of human liver using 16355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16355-1-AP (CYP2C9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP2C9 Polyclonal antibody (16355-1-AP)

    IHC staining of human liver using 16355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16355-1-AP (CYP2C9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse liver tissue, SMMC-7721 cells, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16355-1-AP targets CYP2C9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP2C9 fusion protein Ag9566 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 2, subfamily C, polypeptide 9
Calculated molecular weight162aa,18 kDa; 490aa,56 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC020754
Gene symbol CYP2C9
Gene ID (NCBI) 1559
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP2C9 (Cytochrome P450 Family 2 Subfamily C Member 9) is a crucial cytochrome P450 enzyme, which plays a significant role in the metabolism, by oxidation, of both xenobiotic and endogenous compounds. CYP2C9 is primarily expressed in the liver, and the expression level is reported to be the second highest among CYP isoforms(PMID: 20150829).

Protocols

Product Specific Protocols
WB protocol for CYP2C9 antibody 16355-1-APDownload protocol
IHC protocol for CYP2C9 antibody 16355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols