Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HuH-7 cells, human placenta tissue |
| Positive FC (Intra) detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84378-5-RR targets CYP2R1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CYP2R1 fusion protein Ag12873 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 2, subfamily R, polypeptide 1 |
| Calculated molecular weight | 501 aa, 57 kDa |
| Observed molecular weight | 57 kDa |
| GenBank accession number | BC104909 |
| Gene Symbol | CYP2R1 |
| Gene ID (NCBI) | 120227 |
| RRID | AB_3671915 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6VVX0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CYP2R1 is a member of the cytochrome P450 superfamily of enzymes which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. CYP2R1 primarily functions as a 25-hydroxylase of vitamin D, responsible for converting vitamin D to 25-hydroxyvitamin D (25-OH-D), affecting the production of the active hormone 1,25(OH)2D (PMID: 12867411). CYP2R1 enzyme is a key vitamin D 25-hydroxylase, mutations in CYP2R1 are associated with Rickets vitamin D-dependent 1B(PMID: 25942481, 15128933).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP2R1 antibody 84378-5-RR | Download protocol |
| FC protocol for CYP2R1 antibody 84378-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |