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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP2S1 Polyclonal antibody

CYP2S1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19100-1-AP

Synonyms

Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, EC:1.14.14.-, Cytochrome P450 2S1

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using CYP2S1 Polyclonal antibody (19100-1-AP)

    WB analysis of COLO 320 using 19100-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19100-1-AP (CYP2S1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using CYP2S1 Polyclonal antibody (19100-1-AP)

    WB analysis of mouse kidney using 19100-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19100-1-AP (CYP2S1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using CYP2S1 Polyclonal antibody (19100-1-AP)

    WB analysis of COLO 320 using 19100-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19100-1-AP (CYP2S1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using CYP2S1 Polyclonal antibody (19100-1-AP)

    IHC staining of human ovarian cancer using 19100-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 19100-1-AP (CYP2S1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inCOLO 320 cells, mouse kidney tissue
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

19100-1-AP targets CYP2S1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP2S1 fusion protein Ag4605 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 2, subfamily S, polypeptide 1
Calculated molecular weight 504 aa, 56 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC033691
Gene symbol CYP2S1
Gene ID (NCBI) 29785
RRIDAB_10640528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CYP2S1 antibody 19100-1-APDownload protocol
IHC protocol for CYP2S1 antibody 19100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Pharmacol Rep

Expression of CYP2S1 and CYP2W1 in breast cancer epithelial cells and modulation of their expression by synthetic methoxy stilbenes.

Authors - Hanna Szaefer
View Article