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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP2S1 Monoclonal antibody, PBS Only (Capture)

CYP2S1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E5D4

Cat no : 68198-1-PBS

Synonyms

EC:5.3.99.5, EC:4.2.1.152, EC:1.14.14.-, Cytochrome P450 2S1, CYPIIS1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CYP2S1 Monoclonal antibody (68198-1-Ig)

    WB analysis using 68198-1-Ig (same clone as 68198-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68198-1-Ig (CYP2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68198-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CYP2S1 Monoclonal antibody (68198-1-Ig)

    WB analysis using 68198-1-Ig (same clone as 68198-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68198-1-Ig (CYP2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68198-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50889-4

    Cytometric bead array standard curve of MP50889-4

    Cytometric bead array standard curve of MP50889-4, CYP2S1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68198-1-PBS. Detection antibody: 68198-6-PBS. Standard:Ag4605. Range: 0.391-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68198-1-PBS targets CYP2S1 as part of a matched antibody pair:

MP50889-4: 68198-1-PBS capture and 68198-6-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP2S1 fusion protein Ag4605 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 2, subfamily S, polypeptide 1
Calculated molecular weight 504 aa, 56 kDa
Observed molecular weight50-56 kDa
GenBank accession numberBC033691
Gene symbol CYP2S1
Gene ID (NCBI) 29785
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The cytochrome P450 (CYP) family of isoenzymes are involved in the metabolism of diverse endogenous compounds and detoxification of exogenous agents. Cytochrome P450 2S1 (CYP2S1) is one 'orphan' CYP, and is particularly expressed in epithe-lial cells of tissues which are exposed to the environment such as the skin, respiratory, gastrointes-tinal, and urinary tracts. CYP2S1 is associated primarily with the synthesis and metabo-lism of lipids including prostaglandins (PGE2) and retinoids. In addition, CYP2S1 expression is associated with patient survival in breast cancer.