• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using CYP2W1 Monoclonal antibody (67419-1-Ig)

    WB analysis of HEK-293 using 67419-1-Ig

    WB result of CYP2W1 antibody (67419-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP2W1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using CYP2W1 Monoclonal antibody (67419-1-Ig)

    WB analysis using 67419-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67419-1-Ig (CYP2W1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CYP2W1 Monoclonal antibody (67419-1-Ig)

    IHC staining of human colon cancer using 67419-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67419-1-Ig (CYP2W1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CYP2W1 Monoclonal antibody (67419-1-Ig)

    IF Staining of HepG2 using 67419-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP2W1 antibody (67419-1-Ig, Clone: 2B10H2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HUVEC cells using CYP2W1 Monoclonal antibody (67419-1-Ig)

    FC experiment of HUVEC using 67419-1-Ig

    1x10^6 HUVEC cells were intracellularly stained with 0.4 ug CYP2W1 Monoclonal antibody (67419-1-Ig, Clone:2B10H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

CYP2W1 Monoclonal antibody

CYP2W1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67419-1-Ig
Clone No.2B10H2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

EC:1.14.14.-, Cytochrome P450 2W1, CYPIIW1, 2B10H2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67419-1-Ig targets CYP2W1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP2W1 fusion protein Ag29982 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 2, subfamily W, polypeptide 1
Calculated molecular weight 54 kDa
Observed molecular weight 45-54 kDa
GenBank accession numberBC025761
Gene Symbol CYP2W1
Gene ID (NCBI) 54905
RRIDAB_2882659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8TAV3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytochrome P450s have been proposed to be of importance both in carcinogenesis, by activating precarcinogens, and as determinants of cancer chemotherapy, where they participate in activation or inactivation of anti-cancer drugs. CYP2W1 is exclusively expressed in transformed tissue in the adult human, mainly in colon tumors. It is a potential drug target in cancer therapy. CYP2W1 has a molecular weight of 52-54 kDa, and 30/45 kDa of potential isoforms.

Protocols

Product Specific Protocols
WB protocol for CYP2W1 antibody 67419-1-IgDownload protocol
IHC protocol for CYP2W1 antibody 67419-1-IgDownload protocol
IF protocol for CYP2W1 antibody 67419-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols