Validation Data Gallery
Tested Applications
| Positive WB detected in | rat liver tissue, mouse liver tissue, mouse brain tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 46 publications below |
| IHC | See 9 publications below |
| IF | See 13 publications below |
Product Information
18227-1-AP targets CYP3A4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CYP3A4 fusion protein Ag13074 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 3, subfamily A, polypeptide 4 |
| Calculated molecular weight | 503 aa, 57 kDa |
| Observed molecular weight | 51-57 kDa |
| GenBank accession number | BC101631 |
| Gene Symbol | CYP3A4 |
| Gene ID (NCBI) | 1576 |
| RRID | AB_2090329 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08684 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytochrome P450 3A(CYP3A) belongs to the cytochrome P450 family and the enzymes constitute an important detoxification system that contributes to primary metabolism of more than half of all prescribed medications.CYP3A expression determines impairment of drug absorption and efficient systemic clearance in a tissue-specific manner(PMID:17975676). The highest amounts of drug-metabolizing cytochromes P-450 are found in the liver and intestine, with lower amounts found in other organs, including the kidney, lung, and brain. LPS that has reached the circulation stimulates a TLR4-dependent mechanism in the periphery, evoking a reduction in Cyp3a11 expression and metabolism in the liver (PMID: 15878984). Human CYP3A4 is homologous to five mouse P450 isoforms, namely, Cyp3a11, Cyp3a16, Cyp3a41a, Cyp3a41b, and Cyp3a44 (PMID: 22446519). And CYP3A4 is also the human homologue of rat CYP3A1 and CYP3A2 (PMID: 27546062, PMID: 32874920).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP3A4 antibody 18227-1-AP | Download protocol |
| IHC protocol for CYP3A4 antibody 18227-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions | ||
Hepatology Self-renewal of hepatoblasts under chemically defined conditions by reiterative growth factor and chemical screening. | ||
Acta Biomater Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply. | ||
ACS Appl Mater Interfaces Modeling Colorectal Cancer-Induced Liver Portal Vein Microthrombus on a Hepatic Lobule Chip | ||
Int J Biol Sci Hepatobiliary organoids differentiated from hiPSCs relieve cholestasis-induced liver fibrosis in nonhuman primates | ||
Theranostics Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening. |