CYP3A5 Polyclonal antibody

CYP3A5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13737-1-AP

Synonyms

CP35, CYP3A5, CYPIIIA5, Cytochrome P450 3A5, Cytochrome P450 HLp2, Cytochrome P450 PCN3, P450PCN3, PCN3


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Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13737-1-AP targets CYP3A5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP3A5 fusion protein Ag4674 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 3, subfamily A, polypeptide 5
Calculated molecular weight 502 aa, 57 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC033862
Gene symbol CYP3A5
Gene ID (NCBI) 1577
RRIDAB_2090516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CYP3A5 antibody 13737-1-APDownload protocol
IHC protocol for CYP3A5 antibody 13737-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Oncol

Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.

Authors - Tingbo Ye

Oncotarget

Alpha-ketoglutarate suppresses the NF-κB-mediated inflammatory pathway and enhances the PXR-regulated detoxification pathway.

Authors - Liuqin He
humanWB

Int J Mol Sci

Gene Expression Reprogramming by Citrate Supplementation Reduces HepG2 Cell Migration and Invasion

Authors - Rocchina Miglionico