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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP46A1 Monoclonal antibody, PBS Only

CYP46A1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E10D3

Cat no : 68576-1-PBS

Synonyms

CH24H, Cholesterol 24 hydroxylase, CP46, CYP46, CYP46A1, Cytochrome P450 46A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP46A1 Monoclonal antibody (68576-1-Ig)

    WB analysis using 68576-1-Ig (same clone as 68576-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68576-1-Ig (CYP46A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68576-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CYP46A1 Monoclonal antibody (68576-1-Ig)

    IHC staining of mouse brain using 68576-1-Ig (same clone as 68576-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68576-1-Ig (CYP46A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68576-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CYP46A1 Monoclonal antibody (68576-1-Ig)

    IHC staining of mouse brain using 68576-1-Ig (same clone as 68576-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68576-1-Ig (CYP46A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68576-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68576-1-PBS targets CYP46A1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CYP46A1 fusion protein Ag16786 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 46, subfamily A, polypeptide 1
Calculated molecular weight 500 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC022539
Gene symbol CYP46A1
Gene ID (NCBI) 10858
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cytochrome P450 46A1 (CYP46A1), also named as CYP46, P450 46A1, CH24H and Cholesterol 24-hydroxylase, belongs to the cytochrome P450 family. CYP46A1 is a central nervous system-specific enzyme, and can freely cross the blood-brain barrier and be degraded in the liver. It is involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. CYP46A1 is significantly upregulated in hepatic lymphomyeloid cells compared with ESCs.(PMID:20039312)