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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP4B1 Polyclonal antibody

CYP4B1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11771-1-AP

Synonyms

CYP4B1, CYPIVB1, Cytochrome P450 4B1, Cytochrome P450 HP, P 450HP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP4B1 Polyclonal antibody (11771-1-AP)

    WB analysis using 11771-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11771-1-AP (CYP4B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CYP4B1 Polyclonal antibody (11771-1-AP)

    WB analysis of A549 using 11771-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11771-1-AP (CYP4B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CYP4B1 Polyclonal antibody (11771-1-AP)

    WB analysis of mouse lung using 11771-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11771-1-AP (CYP4B1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CYP4B1 Polyclonal antibody (11771-1-AP)

    WB analysis of mouse lung using 11771-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11771-1-AP (CYP4B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using CYP4B1 Polyclonal antibody (11771-1-AP)

    IP experiment of mouse lung using 11771-1-AP

    IP result of anti-CYP4B1 (IP:11771-1-AP, 4ug; Detection:11771-1-AP 1:500) with mouse lung tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CYP4B1 Polyclonal antibody (11771-1-AP)

    IHC staining of human lung cancer using 11771-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11771-1-AP (CYP4B1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inmouse lung tissue, A549 cells, rat kidney tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

11771-1-AP targets CYP4B1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP4B1 fusion protein Ag2416 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 4, subfamily B, polypeptide 1
Calculated molecular weight 512 aa, 59 kDa
Observed molecular weight 55-59 kDa
GenBank accession numberBC017758
Gene symbol CYP4B1
Gene ID (NCBI) 1580
RRIDAB_11182944
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CYP4B1 antibody 11771-1-APDownload protocol
IHC protocol for CYP4B1 antibody 11771-1-APDownload protocol
IP protocol for CYP4B1 antibody 11771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Hum Reprod

Single-cell profiling reveals mechanisms of uncontrolled inflammation and glycolysis in decidual stromal cell subtypes in recurrent miscarriage

Authors - Shihua Bao
View Article