Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of MCF-7 using 12286-1-AP
WB result of CYP4F11 antibody (12286-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP4F11 transfected MCF-7 cells.
WB analysis of human liver using 12286-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 12286-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 12286-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 12286-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12286-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 12286-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12286-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 12286-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12286-1-AP (CYP4F11 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 12286-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CYP4F11 antibody (12286-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

CYP4F11 Polyclonal antibody

CYP4F11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12286-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Synonyms

EC:1.14.14.78, EC:1.14.14.1, Docosahexaenoic acid omega-hydroxylase, Cytochrome P450 4F11, CYPIVF11

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, human liver tissue, human kidney tissue, MCF-7 cells, mouse liver tissue
Positive IHC detected in	human liver cancer tissue, human bowen disease **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
ChIP	See 1 publications below

Product Information

12286-1-AP targets CYP4F11 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CYP4F11 fusion protein Ag2906 相同性解析による交差性が予測される生物種
Full Name	cytochrome P450, family 4, subfamily F, polypeptide 11
Calculated molecular weight	524 aa, 60 kDa
Observed molecular weight	57 kDa
GenBank accession number	BC016853
Gene Symbol	CYP4F11
Gene ID (NCBI)	57834
RRID	AB_2088407
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9HBI6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CYP4F11, also named as CYPIVF11, belongs to the cytochrome P450 family. It is the lone CYP4F/A enzyme that effectively oxidized 3-hydroxypalmitate. It is the predominant catalyst of this fatty acid oxidation reaction in human liver. CYP4F11 is one of the primary human epidermal keratinocytes. It is positively regulated by multiple signaling pathways in HaCaT keratinocytes, including RXR and JNK signaling pathways. (PMID:19812349)

Protocols

Product Specific Protocols
WB protocol for CYP4F11 antibody 12286-1-AP	Download protocol
IHC protocol for CYP4F11 antibody 12286-1-AP	Download protocol
IF protocol for CYP4F11 antibody 12286-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Drug Metab Dispos Gene regulation of CYP4F11 in human keratinocyte HaCaT cells. Authors - Wang Ying Y View Article
human	ChIP	Drug Metab Dispos Regulation of cytochrome P450 4F11 by nuclear transcription factor-κB. Authors - Bell Jordan C JC View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MCF-7 using 12286-1-AP

WB analysis of human liver using 12286-1-AP

WB analysis using 12286-1-AP

WB analysis using 12286-1-AP

WB analysis of human liver using 12286-1-AP

WB analysis of human kidney using 12286-1-AP

IHC staining of human liver cancer using 12286-1-AP