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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using CYP4F3 Polyclonal antibody (19701-1-AP)

    WB analysis of HL-60 using 19701-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19701-1-AP (CYP4F3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CYP4F3 Polyclonal antibody

CYP4F3 Polyclonal Antibody for WB, ELISA
Cat No. 19701-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CPF3, CYP4F, CYP4F3, CYPIVF3, Cytochrome P450 4F3, Cytochrome P450 LTB omega, LTB4H

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19701-1-AP targets CYP4F3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 4, subfamily F, polypeptide 3
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberNM_000896
Gene Symbol CYP4F3
Gene ID (NCBI) 4051
RRIDAB_2878599
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08477
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP4F3, also named as LTB4H and CYPIVF3, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. CYP4F3 requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4, a potent chemoattractant for polymorphonuclear leukocytes.

Protocols

Product Specific Protocols
WB protocol for CYP4F3 antibody 19701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols