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CYP51A1 Polyclonal antibody
CYP51A1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 13431-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, mouse testis tissue, rat liver tissue, HuH-7 cells |
| Positive IP detected in | mouse testis tissue, mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 25 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
13431-1-AP targets CYP51A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CYP51A1 fusion protein Ag4235 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 51, subfamily A, polypeptide 1 |
| Calculated molecular weight | 509 aa, 57 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC032322 |
| Gene symbol | CYP51A1 |
| Gene ID (NCBI) | 1595 |
| RRID | AB_2088571 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lanosterol 14-alpha demethylase (cytochrome P450(51), CYP51A1) belongs to the evolutionarily conserved family of cytochrome P450 and catalyzes one of the key steps in cholesterol biosynthesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP51A1 antibody 13431-1-AP | Download protocol |
| IF protocol for CYP51A1 antibody 13431-1-AP | Download protocol |
| IP protocol for CYP51A1 antibody 13431-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions | ||
Mol Cell Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex | ||
Mol Cell Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex. | ||
Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Hua (Verified Customer) (12-01-2020) | Clear band with clean background
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