CYP7A1 Polyclonal antibody

CYP7A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18054-1-AP

Synonyms

Cytochrome P450 7A1, CYPVII, CYP7, CP7A, Cholesterol 7-alpha-monooxygenase


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Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells, L02 cells, SMMC-7721 cells
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18054-1-AP targets CYP7A1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP7A1 fusion protein Ag12572 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 7, subfamily A, polypeptide 1
Calculated molecular weight 504 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC101777
Gene symbol CYP7A1
Gene ID (NCBI) 1581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP7A1 (Cytochrome P450 Family 7 Subfamily A Member 1), is a cholesterol 7alpha-hydroxylase, catalyzing the first and rate-limiting step in the neutral or classic pathway for bile acid biosynthesis. CYP7A1 is a postulated gene modifier of colorectal cancer risk and target for the therapeutic bile acid, ursodeoxycholic acid (UDCA). The CYP7A1 enzyme controls the rate-limiting step of the catabolism of cholesterol into bile acids in the liver.

Protocols

Product Specific Protocols
WB protocol for CYP7A1 antibody 18054-1-APDownload protocol
IHC protocol for CYP7A1 antibody 18054-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Mol Gastroenterol Hepatol

Endoplasmic Reticulum Stress Regulates Hepatic Bile Acid Metabolism in Mice.

Authors - Anne S Henkel
humanWB

Cell Mol Gastroenterol Hepatol

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

Authors - Dayan Sun
mouseWB

J Ethnopharmacol

Multi-omics and chemical profiling approaches to understand the material foundation and pharmacological mechanism of Sophorae Tonkinensis Radix et Rhizome-induced liver injury in mice

Authors - Si-Wei Rao
MOUSEWB

J Lipid Res

Hepatic Deletion of X-box Binding Protein 1 Impairs Bile Acid Metabolism in Mice.

Authors - Xiaoying Liu
mouseWB

Toxicol Sci

Hepatic, metabolic and toxicity evaluation of repeated oral administration of SnS2 nanoflowers in mice.

Authors - Disi Bai
humanIHC

J Steroid Biochem Mol Biol

Bird's eye view analysis of in situ cholesterol metabolic pathways in breast cancer patients and its clinicopathological significance in their subtypes.

Authors - Shinkichi Kosaka