CYP7B1 Polyclonal antibody

Name: CYP7B1 Polyclonal antibody
Brand: Proteintech
SKU: 24889-1-AP

CYP7B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 24889-1-AP

Synonyms

CBAS3, CP7B, CYP7B1, Cytochrome P450 7B1, Oxysterol 7 alpha hydroxylase, SPG5A

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of A431 using 24889-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 24889-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 24889-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse liver using 24889-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 24889-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 24889-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of mouse liver using 24889-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 24889-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 24889-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 24889-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 24889-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 24889-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 24889-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 24889-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HEK-293 using 24889-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HEK-293 using 24889-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 24889-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HEK-293 cells, mouse liver tissue
Positive IHC detected in	human liver cancer tissue, human brain tissue, human liver tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 23 publications below
IHC	See 2 publications below

Product Information

24889-1-AP targets CYP7B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CYP7B1 fusion protein Ag19374 相同性解析による交差性が予測される生物種
Full Name	cytochrome P450, family 7, subfamily B, polypeptide 1
Calculated molecular weight	506 aa, 58 kDa
Observed molecular weight	50-55 kDa
GenBank accession number	BC136574
Gene symbol	CYP7B1
Gene ID (NCBI)	9420
RRID	AB_2879780
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.

Protocols

Product Specific Protocols
WB protocol for CYP7B1 antibody 24889-1-AP	Download protocol
IHC protocol for CYP7B1 antibody 24889-1-AP	Download protocol
IF protocol for CYP7B1 antibody 24889-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse,rat	WB	Free Radic Biol Med ALDH2 deficiency exacerbates MCD-diet induced MASLD by modulating bile acid metabolism Authors - Jun Luo View Article
mouse	WB	Cell Physiol Biochem Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF. Authors - Dandan Zhong View Article
mouse	WB	Mol Nutr Food Res The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation Authors - Mengcheng Jin View Article
mouse	WB	J Nutr Biochem Deficiency of coiled-coil domain containing 80 increases plasma cholesterol by decreasing fecal sterols excretion in hypercholesterolemic mice. Authors - Wenyun Li View Article
mouse	WB	Biochem Pharmacol Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity. Authors - Yanan Chen View Article
mouse	WB	J Lipid Res Insulin resistance dysregulates CYP7B1 leading to oxysterol accumulation: a pathway for NAFL to NASH transition. Authors - Genta Kakiyama View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP7B1 Polyclonal antibody

CYP7B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of A431 using 24889-1-AP

WB analysis of HEK-293 using 24889-1-AP

WB analysis of mouse liver using 24889-1-AP

IHC staining of mouse liver using 24889-1-AP

IHC staining of human liver cancer using 24889-1-AP

IHC staining of human liver cancer using 24889-1-AP

IHC staining of mouse liver using 24889-1-AP

IHC staining of human liver using 24889-1-AP

IHC staining of human liver using 24889-1-AP

IHC staining of human brain using 24889-1-AP

IHC staining of human brain using 24889-1-AP

IHC staining of human liver cancer using 24889-1-AP

IHC staining of human liver cancer using 24889-1-AP

IF Staining of HeLa using 24889-1-AP

IF Staining of HEK-293 using 24889-1-AP

IF Staining of HEK-293 using 24889-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ALDH2 deficiency exacerbates MCD-diet induced MASLD by modulating bile acid metabolism

Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF.

The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation

Deficiency of coiled-coil domain containing 80 increases plasma cholesterol by decreasing fecal sterols excretion in hypercholesterolemic mice.

Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity.

Insulin resistance dysregulates CYP7B1 leading to oxysterol accumulation: a pathway for NAFL to NASH transition.