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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP7B1 Recombinant antibody

CYP7B1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230160H5

Cat no : 82927-1-RR

Synonyms

CP7B, CBAS3, 3-hydroxysteroid 7-alpha hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase, 24-hydroxycholesterol 7-alpha-hydroxylase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP7B1 Recombinant antibody (82927-1-RR)

    WB analysis using 82927-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82927-1-RR (CYP7B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CYP7B1 Recombinant antibody (82927-1-RR)

    IF Staining of HepG2 using 82927-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP7B1 antibody (82927-1-RR, Clone: 230160H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CYP7B1 Recombinant antibody (82927-1-RR)

    IF Staining of HEK-293 using 82927-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (82927-1-RR, Clone: 230160H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using CYP7B1 Recombinant antibody (82927-1-RR)

    FC experiment of HeLa using 82927-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CYP7B1 Recombinant antibody (82927-1-RR, Clone:230160H5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells, HeLa cells, HCT 116 cells
Positive IF/ICC detected inHepG2 cells, HEK-293 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82927-1-RR targets CYP7B1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CYP7B1 fusion protein Ag19374 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 7, subfamily B, polypeptide 1
Calculated molecular weight 506 aa, 58 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC136574
Gene symbol CYP7B1
Gene ID (NCBI) 9420
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.

Protocols

Product Specific Protocols
WB protocol for CYP7B1 antibody 82927-1-RRDownload protocol
IF protocol for CYP7B1 antibody 82927-1-RRDownload protocol
FC protocol for CYP7B1 antibody 82927-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols