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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYR61/CCN1 Monoclonal antibody, PBS Only

CYR61/CCN1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E11F11

Cat no : 67656-1-PBS

Synonyms

CYR61, Cysteine-rich angiogenic inducer 61, Cellular communication network factor 1, CCN1, CCN family member 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYR61/CCN1 Monoclonal antibody (67656-1-Ig)

    WB analysis using 67656-1-Ig (same clone as 67656-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67656-1-Ig (CYR61 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYR61/CCN1 Monoclonal antibody (67656-1-Ig)

    IHC staining of human liver cancer using 67656-1-Ig (same clone as 67656-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYR61/CCN1 Monoclonal antibody (67656-1-Ig)

    IHC staining of human liver cancer using 67656-1-Ig (same clone as 67656-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CYR61/CCN1 Monoclonal antibody (67656-1-Ig)

    IHC staining of human stomach cancer using 67656-1-Ig (same clone as 67656-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CYR61/CCN1 Monoclonal antibody (67656-1-Ig)

    IHC staining of human stomach cancer using 67656-1-Ig (same clone as 67656-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CYR61/CCN1 Monoclonal antibody (67656-1-Ig)

    IF Staining of MCF-7 using 67656-1-Ig (same clone as 67656-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CYR61 antibody (67656-1-Ig, Clone: 2E11F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CYR61/CCN1 Monoclonal antibody (67656-1-Ig)

    IF Staining of A431 using 67656-1-Ig (same clone as 67656-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CYR61 antibody (67656-1-Ig, Clone: 2E11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67656-1-PBS targets CYR61/CCN1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYR61/CCN1 fusion protein Ag25129 相同性解析による交差性が予測される生物種
Full Name cysteine-rich, angiogenic inducer, 61
Calculated molecular weight 42 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberBC001271
Gene symbol CYR61
Gene ID (NCBI) 3491
RRIDAB_2882854
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.