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  • Featured Product
  • KD/KO Validated

CaMKII Gamma Polyclonal antibody, PBS Only

CaMKII Gamma Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12666-2-PBS

Synonyms

CAMK2G, CaMK-II subunit gamma, CAMK-II, CAMK, CaM kinase II subunit gamma

Validation Data Gallery

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  • Western Blot (WB) analysis of SMMC-7721 cells using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    WB analysis of SMMC-7721 using 12666-2-AP (same clone as 12666-2-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    WB analysis of human brain using 12666-2-AP (same clone as 12666-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    WB analysis of SMMC-7721 using 12666-2-AP (same clone as 12666-2-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    IP experiment of mouse heart using 12666-2-AP (same clone as 12666-2-PBS)

    IP result of anti-CaMKII Gamma (IP:12666-2-AP, 4ug; Detection:12666-2-AP 1:1000) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    IHC staining of mouse heart using 12666-2-AP (same clone as 12666-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12666-2-AP (CaMKII Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    IHC staining of human pancreas cancer using 12666-2-AP (same clone as 12666-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12666-2-AP (CaMKII gamma antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    IF Staining of HepG2 using 12666-2-AP (same clone as 12666-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CaMKII Gamma antibody (12666-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12666-2-PBS targets CaMKII Gamma in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CaMKII Gamma fusion protein Ag3348 相同性解析による交差性が予測される生物種
Full Name calcium/calmodulin-dependent protein kinase II gamma
Calculated molecular weight 527 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC034044
Gene symbol CAMK2G
Gene ID (NCBI) 818
RRIDAB_2068213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CaMKII gamma, also named CAMKG or CAMK2G, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G is expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some studies can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes(PMID: 12032636). CAMK2G has some isoforms with the MW of 55-66 kDa and this antibody can recognize all the isoforms of CAMK2G. This antibody may have cross-reaction with CAMK2A/B/D due to the high homology.