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  • Featured Product
  • KD/KO Validated

CaMKII Gamma Polyclonal antibody, PBS Only

CaMKII Gamma Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12666-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of SMMC-7721 cells using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    WB analysis of SMMC-7721 using 12666-2-AP (same clone as 12666-2-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    WB analysis of SMMC-7721 using 12666-2-AP (same clone as 12666-2-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    WB analysis of human brain using 12666-2-AP (same clone as 12666-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    IP experiment of mouse heart using 12666-2-AP (same clone as 12666-2-PBS)

    IP result of anti-CaMKII Gamma (IP:12666-2-AP, 4ug; Detection:12666-2-AP 1:1000) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    IHC staining of mouse heart using 12666-2-AP (same clone as 12666-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12666-2-AP (CaMKII Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    IHC staining of human pancreas cancer using 12666-2-AP (same clone as 12666-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12666-2-AP (CaMKII gamma antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CaMKII Gamma Polyclonal antibody (12666-2-AP)

    IF Staining of HepG2 using 12666-2-AP (same clone as 12666-2-PBS)

    Immunofluorescent analysis of HepG2 cells, using CAMK2G antibody 12666-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12666-2-PBS targets CaMKII Gamma in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CaMKII Gamma fusion protein Ag3348 相同性解析による交差性が予測される生物種
Full Name calcium/calmodulin-dependent protein kinase II gamma
Calculated molecular weight 527 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC034044
Gene symbol CAMK2G
Gene ID (NCBI) 818
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CaMKII gamma, also named CAMKG or CAMK2G, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G is expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some studies can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes(PMID: 12032636). CAMK2G has some isoforms with the MW of 55-66 kDa and this antibody can recognize all the isoforms of CAMK2G. This antibody may have cross-reaction with CAMK2A/B/D due to the high homology.