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Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    WB analysis of human colon using 60351-1-Ig (same clone as 60351-1-PBS)

    Untreated and PNGase F-treated lysates of human colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    WB analysis of pig colon using 60351-1-Ig (same clone as 60351-1-PBS)

    pig colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    WB analysis of human colon using 60351-1-Ig (same clone as 60351-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon using 60351-1-Ig (same clone as 60351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon using 60351-1-Ig (same clone as 60351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human stomach cancer using 60351-1-Ig (same clone as 60351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human stomach cancer using 60351-1-Ig (same clone as 60351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human pancreas cancer using 60351-1-Ig (same clone as 60351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human pancreas cancer using 60351-1-Ig (same clone as 60351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IF Staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IF Staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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Cadherin-17 Monoclonal antibody, PBS Only

Cadherin-17 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60351-1-PBS
Clone No.3B10A9

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CDH17, HPT1, HPT 1, CDH16, Cadherin 17

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60351-1-PBS targets Cadherin-17 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cadherin-17 fusion protein Ag19488 相同性解析による交差性が予測される生物種
Full Name cadherin 17, LI cadherin (liver-intestine)
Calculated molecular weight 832 aa, 92 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC113464
Gene Symbol Cadherin-17
Gene ID (NCBI) 1015
RRIDAB_2881460
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ12864
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.