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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cadherin-17 Recombinant antibody

Cadherin-17 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240681B11

Cat no : 83793-5-RR

Synonyms

CDH17, HPT1, HPT 1, CDH16, Cadherin 17

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using Cadherin-17 Recombinant antibody (83793-5-RR)

    WB analysis of mouse colon using 83793-5-RR

    mouse colon tissue was subjected to SDS PAGE followed by western blot with 83793-5-RR (Cadherin-17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Cadherin-17 Recombinant antibody (83793-5-RR)

    IHC staining of mouse colon using 83793-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Cadherin-17 Recombinant antibody (83793-5-RR)

    IHC staining of mouse colon using 83793-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83793-5-RR

    Affinity and Kinetic Characterization of 83793-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83793-5-RR against Human Cadherin-17 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inmouse colon tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83793-5-RR is Cadherin-17 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cadherin-17 fusion protein Ag19488 相同性解析による交差性が予測される生物種
Full Name cadherin 17, LI cadherin (liver-intestine)
Calculated molecular weight 832 aa, 92 kDa
Observed molecular weight120 kDa
GenBank accession numberBC113464
Gene symbol Cadherin-17
Gene ID (NCBI) 1015
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherin-17, also known as LI-cadherin, is a member of the cadherin superfamily. Cadherins are transmembrane proteins that mediates cell-cell adhesion, plays important roles during embryogenesis, and is crucial for tissue morphogenesis and maintenance. Cadherin-17 is a component of the gastrointestinal tract and pancreatic ducts and is involved in intestinal peptide transport. It may also play a role in the morphological organization of liver and intestine.

Protocols

Product Specific Protocols
WB protocol for Cadherin-17 antibody 83793-5-RRDownload protocol
IHC protocol for Cadherin-17 antibody 83793-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols