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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calmodulin 1/2/3 Polyclonal antibody

Calmodulin 1/2/3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28270-1-AP

Synonyms

CALM, CALM1, CALM2, CALM3, CALML2, Calmodulin, Calmodulin 1/2/3, CaM, CAM1, CAM2, CAM3, CAMB, CAMC, CAMII, CAMIII, PHKD, PHKD2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Calmodulin 1/2/3 Polyclonal antibody (28270-1-AP)

    WB analysis using 28270-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Calmodulin 1/2/3 Polyclonal antibody (28270-1-AP)

    IHC staining of rat brain using 28270-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using Calmodulin 1/2/3 Polyclonal antibody (28270-1-AP)

    IHC staining of rat brain using 28270-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Calmodulin 1/2/3 Polyclonal antibody (28270-1-AP)

    IHC staining of mouse brain using 28270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Calmodulin 1/2/3 Polyclonal antibody (28270-1-AP)

    IHC staining of mouse skeletal muscle using 28270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Calmodulin 1/2/3 Polyclonal antibody (28270-1-AP)

    IF Staining of HeLa using 28270-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calmodulin 1/2/3 antibody (28270-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inrat brain tissue, mouse brain tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28270-1-AP targets Calmodulin 1/2/3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calmodulin 1/2/3 fusion protein Ag28000 相同性解析による交差性が予測される生物種
Full Name calmodulin 2 (phosphorylase kinase, delta)
Calculated molecular weight 17 kDa
Observed molecular weight 17-22 kDa
GenBank accession numberBC006464
Gene symbol Calmodulin 1
Gene ID (NCBI) 805
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calmodulin is the most widely distributed and most versatile member of a family of calcium-binding proteins which probably serve as receptors for the Ca2+ signal. Through the regulation of a wide variety of intracellular enzymes, calmodulin plays a key role in many physiological processes (PMID: 6313166).

Protocols

Product Specific Protocols
WB protocol for Calmodulin 1/2/3 antibody 28270-1-APDownload protocol
IHC protocol for Calmodulin 1/2/3 antibody 28270-1-APDownload protocol
IF protocol for Calmodulin 1/2/3 antibody 28270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols