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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Calnexin Monoclonal antibody (66903-1-Ig)

    WB analysis of HEK-293 using 66903-1-Ig (same clone as 66903-1-PBS)

    WB result of Calnexin antibody (66903-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calnexin transfected HEK-293 cells. This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Calnexin Monoclonal antibody (66903-1-Ig)

    WB analysis using 66903-1-Ig (same clone as 66903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66903-1-Ig (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Calnexin Monoclonal antibody (66903-1-Ig)

    WB analysis of HEK-293 using 66903-1-Ig (same clone as 66903-1-PBS)

    HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 66903-1-Ig (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Calnexin Monoclonal antibody (66903-1-Ig)

    IHC staining of human hepatocirrhosis using 66903-1-Ig (same clone as 66903-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66903-1-Ig (Calnexin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Calnexin Monoclonal antibody (66903-1-Ig)

    IHC staining of human hepatocirrhosis using 66903-1-Ig (same clone as 66903-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66903-1-Ig (Calnexin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Calnexin Monoclonal antibody (66903-1-Ig)

    IF Staining of HeLa using 66903-1-Ig (same clone as 66903-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66903-1-Ig (Calnexin antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). Red: staning with CoraLite555-Phalloidin. This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Calnexin Monoclonal antibody (66903-1-Ig)

    IF Staining of HepG2 using 66903-1-Ig (same clone as 66903-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66903-1-Ig (Calnexin antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Calnexin Monoclonal antibody, PBS Only

Calnexin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66903-1-PBS
Clone No.2A2C6

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66903-1-PBS targets Calnexin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calnexin fusion protein Ag25535 相同性解析による交差性が予測される生物種
Full Name calnexin
Calculated molecular weight 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC003552
Gene Symbol Calnexin
Gene ID (NCBI) 821
RRIDAB_2882231
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27824
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.