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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calnexin Recombinant antibody, PBS Only

Calnexin Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K18

Cat no : 81938-1-PBS

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Calnexin Recombinant antibody (81938-1-RR)

    WB analysis using 81938-1-RR (same clone as 81938-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81938-1-RR (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Calnexin Recombinant antibody (81938-1-RR)

    IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81938-1-RR (Calnexin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Calnexin Recombinant antibody (81938-1-RR)

    IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81938-1-RR (Calnexin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Calnexin Recombinant antibody (81938-1-RR)

    IF Staining of HepG2 using 81938-1-RR (same clone as 81938-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (81938-1-RR, Clone: 3K18 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Calnexin Recombinant antibody (81938-1-RR)

    IF Staining of HepG2 using 81938-1-RR (same clone as 81938-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (81938-1-RR, Clone: 3K18 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81938-1-PBS

    Affinity and Kinetic Characterization of 81938-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 81938-1-RR against Human Calnexin were performed. The affinity constant is 1.76 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81938-1-PBS targets Calnexin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Calnexin fusion protein Ag0697 相同性解析による交差性が予測される生物種
Full Name calnexin
Calculated molecular weight 90 kDa
Observed molecular weight90 kDa
GenBank accession numberBC003552
Gene symbol Calnexin
Gene ID (NCBI) 821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.