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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Calpain 3 Polyclonal antibody (10415-1-AP)

    WB analysis of mouse skeletal muscle using 10415-1-AP (same clone as 10415-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10415-1-AP (Calpain 3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Calpain 3 Polyclonal antibody (10415-1-AP)

    WB analysis of mouse skeletal muscle using 10415-1-AP (same clone as 10415-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10415-1-AP (Calpain 3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Calpain 3 Polyclonal antibody (10415-1-AP)

    IHC staining of human urothelial carcinoma using 10415-1-AP (same clone as 10415-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10415-1-AP (Calpain 3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Calpain 3 Polyclonal antibody (10415-1-AP)

    IHC staining of human urothelial carcinoma using 10415-1-AP (same clone as 10415-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10415-1-AP (Calpain 3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

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Calpain 3 Polyclonal antibody, PBS Only

Calpain 3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10415-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10415-1-PBS targets Calpain 3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calpain 3 fusion protein Ag0671 相同性解析による交差性が予測される生物種
Full Name calpain 3, (p94)
Calculated molecular weight 94 kDa
Observed molecular weight 90-105 kDa, 55-60 kDa, 30-35 kDa
GenBank accession numberBC003521
Gene Symbol CAPN3
Gene ID (NCBI) 825
RRIDAB_2069647
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20807
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).