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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Calpastatin Monoclonal antibody (67107-1-Ig)

    WB analysis of HeLa using 67107-1-Ig (same clone as 67107-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (CAST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Calpastatin Monoclonal antibody (67107-1-Ig)

    WB analysis of HepG2 using 67107-1-Ig (same clone as 67107-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using Calpastatin Monoclonal antibody (67107-1-Ig)

    WB analysis of LNCaP using 67107-1-Ig (same clone as 67107-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Calpastatin Monoclonal antibody (67107-1-Ig)

    WB analysis of MCF-7 using 67107-1-Ig (same clone as 67107-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Calpastatin Monoclonal antibody (67107-1-Ig)

    WB analysis of HEK-293 using 67107-1-Ig (same clone as 67107-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using Calpastatin Monoclonal antibody (67107-1-Ig)

    WB analysis of THP-1 using 67107-1-Ig (same clone as 67107-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Calpastatin Monoclonal antibody (67107-1-Ig)

    IF Staining of HeLa using 67107-1-Ig (same clone as 67107-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calpastatin antibody (67107-1-Ig, Clone: 1G12A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Calpastatin Monoclonal antibody (67107-1-Ig)

    FC experiment of HeLa using 67107-1-Ig (same clone as 67107-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Calpastatin Monoclonal antibody (67107-1-Ig, Clone:1G12A2) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50744-2

    Cytometric bead array standard curve of MP50744-2

    Cytometric bead array standard curve of MP50744-2, CAST Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67107-1-PBS. Detection antibody: 67107-3-PBS. Standard:Ag28669. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50744-3

    Cytometric bead array standard curve of MP50744-3

    Cytometric bead array standard curve of MP50744-3, CAST Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67107-1-PBS. Detection antibody: 67107-4-PBS. Standard:Ag28669. Range: 0.098-100 ng/mL.

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Calpastatin Monoclonal antibody, PBS Only (Capture)

Calpastatin Monoclonal Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 67107-1-PBS
Clone No.1G12A2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

CAST, Sperm BS-17 component, Calpain inhibitor, 1G12A2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67107-1-PBS targets Calpastatin as part of a matched antibody pair:

MP50744-2: 67107-1-PBS capture and 67107-3-PBS detection (validated in Cytometric bead array)

MP50744-3: 67107-1-PBS capture and 67107-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calpastatin fusion protein Ag28669 相同性解析による交差性が予測される生物種
Full Name calpastatin
Calculated molecular weight 667 aa, 72 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC013579
Gene Symbol Calpastatin
Gene ID (NCBI) 831
RRIDAB_2882411
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20810
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.