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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calponin Polyclonal antibody, PBS Only

Calponin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24855-1-PBS

Synonyms

CNN1, CNN 1, Calponin-1, Calponin 1, Basic calponin

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse stomach tissue using Calponin Polyclonal antibody (24855-1-AP)

    WB analysis of mouse stomach using 24855-1-AP (same clone as 24855-1-PBS)

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24855-1-AP (Calponin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Calponin Polyclonal antibody (24855-1-AP)

    IHC staining of human colon using 24855-1-AP (same clone as 24855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24855-1-AP (Calponin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Calponin Polyclonal antibody (24855-1-AP)

    IHC staining of human heart using 24855-1-AP (same clone as 24855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Calponin Polyclonal antibody (24855-1-AP)

    IHC staining of human heart using 24855-1-AP (same clone as 24855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using Calponin Polyclonal antibody (24855-1-AP)

    IHC staining of human breast hyperplasia using 24855-1-AP (same clone as 24855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using Calponin Polyclonal antibody (24855-1-AP)

    IHC staining of human skeletal muscle using 24855-1-AP (same clone as 24855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using Calponin Polyclonal antibody (24855-1-AP)

    IHC staining of human skeletal muscle using 24855-1-AP (same clone as 24855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Calponin Polyclonal antibody (24855-1-AP)

    IF Staining of mouse heart using 24855-1-AP (same clone as 24855-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calponin antibody (24855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using Calponin Polyclonal antibody (24855-1-AP)

    IF Staining of C2C12 using 24855-1-AP (same clone as 24855-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Calponin antibody (24855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24855-1-PBS targets Calponin in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calponin fusion protein Ag21384 相同性解析による交差性が予測される生物種
Full Name calponin 1, basic, smooth muscle
Calculated molecular weight 33 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC022015
Gene symbol Calponin
Gene ID (NCBI) 1264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kDa protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.