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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    WB analysis of human heart using 18422-1-AP (same clone as 18422-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    WB analysis using 18422-1-AP (same clone as 18422-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    WB analysis of human skeletal muscle using 18422-1-AP (same clone as 18422-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    IHC staining of human heart using 18422-1-AP (same clone as 18422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18422-1-AP (Calsequestrin 2 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    IHC staining of human heart using 18422-1-AP (same clone as 18422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18422-1-AP (Calsequestrin 2 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    IHC staining of human placenta using 18422-1-AP (same clone as 18422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    IHC staining of human skin using 18422-1-AP (same clone as 18422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    IHC staining of human skin using 18422-1-AP (same clone as 18422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    IHC staining of human spleen using 18422-1-AP (same clone as 18422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    IHC staining of human spleen using 18422-1-AP (same clone as 18422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    IHC staining of human kidney using 18422-1-AP (same clone as 18422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    IHC staining of human kidney using 18422-1-AP (same clone as 18422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    IHC staining of human placenta using 18422-1-AP (same clone as 18422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    IHC staining of human ovary using 18422-1-AP (same clone as 18422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    IHC staining of human ovary using 18422-1-AP (same clone as 18422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using Calsequestrin 2 Polyclonal antibody (18422-1-AP)

    IF Staining of C2C12 using 18422-1-AP (same clone as 18422-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed C2C12 cell using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18422-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18422-1-PBS targets Calsequestrin 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calsequestrin 2 fusion protein Ag13246 相同性解析による交差性が予測される生物種
Full Name calsequestrin 2 (cardiac muscle)
Calculated molecular weight 46 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC022288
Gene Symbol Calsequestrin 2
Gene ID (NCBI) 845
RRIDAB_2071473
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14958
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.