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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calsequestrin 2 Monoclonal antibody, PBS Only

Calsequestrin 2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C10A1

Cat no : 66419-1-PBS

Synonyms

Calsequestrin 2, CASQ2, PDIB2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Calsequestrin 2 Monoclonal antibody (66419-1-Ig)

    WB analysis using 66419-1-Ig (same clone as 66419-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Calsequestrin 2 Monoclonal antibody (66419-1-Ig)

    WB analysis using 66419-1-Ig (same clone as 66419-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using Calsequestrin 2 Monoclonal antibody (66419-1-Ig)

    WB analysis of rat skeletal muscle using 66419-1-Ig (same clone as 66419-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Calsequestrin 2 Monoclonal antibody (66419-1-Ig)

    WB analysis of mouse skeletal muscle using 66419-1-Ig (same clone as 66419-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Calsequestrin 2 Monoclonal antibody (66419-1-Ig)

    IHC staining of human heart using 66419-1-Ig (same clone as 66419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Calsequestrin 2 Monoclonal antibody (66419-1-Ig)

    IHC staining of human heart using 66419-1-Ig (same clone as 66419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Calsequestrin 2 Monoclonal antibody (66419-1-Ig)

    IF Staining of mouse heart using 66419-1-Ig (same clone as 66419-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calsequestrin 2 antibody (66419-1-Ig, Clone: 1C10A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Calsequestrin 2 Monoclonal antibody (66419-1-Ig)

    IF Staining of mouse heart using 66419-1-Ig (same clone as 66419-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calsequestrin 2 antibody (66419-1-Ig, Clone: 1C10A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66419-1-PBS targets Calsequestrin 2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig, mouse samples.

Tested Reactivity human, rat, pig, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Calsequestrin 2 fusion protein Ag13246 相同性解析による交差性が予測される生物種
Full Name calsequestrin 2 (cardiac muscle)
Calculated molecular weight 46 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC022288
Gene symbol CASQ2
Gene ID (NCBI) 845
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.